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Assay Detail

CHEMBL750485

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity for nAChR with [3H]-nicotine in rat alpha4 beta2 membranes
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor subunit alpha-4 (CHEMBL307)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

5-Azidoimidacloprid and an acyclic analogue as candidate photoaffinity probes for mammalian and insect nicotinic acetylcholine receptors.
Kagabu S, Maienfisch P, Zhang A, Granda-Minones J, Haettenschwiler J, Kayser H, Maetzke T, Casida JE.
J Med Chem (2000) 43 : 5003 -5009
PubMed 11150171 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 7.83 8.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL97343 5-AZIDOIMIDACLOPRID 1 8.36
CHEMBL406819 IMIDACLOPRID 1 7.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL97343 5-AZIDOIMIDACLOPRID Ki = 4.4 nM 8.36
CHEMBL406819 IMIDACLOPRID Ki = 50.0 nM 7.30