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Assay Detail

CHEMBL751222

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]nicotine binding to Nicotinic acetylcholine receptor alpha4-beta2 in rat brain
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Development of subtype-selective ligands as antagonists at nicotinic receptors mediating nicotine-evoked dopamine release.
Crooks PA, Ayers JT, Xu R, Sumithran SP, Grinevich VP, Wilkins LH, Deaciuc AG, Allen DD, Dwoskin LP.
Bioorg Med Chem Lett (2004) 14 : 1869 -1874
PubMed 15050618 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 5.51 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL56296 1 7.05
CHEMBL55606 1 6.85
CHEMBL54826 1 5.71
CHEMBL54580 1 5.04
CHEMBL55297 1 4.77
CHEMBL293527 1 4.70
CHEMBL55479 1 4.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL56296 Ki = 90.0 nM 7.05
CHEMBL55606 Ki = 140.0 nM 6.85
CHEMBL54826 Ki = 1950.0 nM 5.71
CHEMBL54580 Ki = 9150.0 nM 5.04
CHEMBL55297 Ki = 17000.0 nM 4.77
CHEMBL293527 Ki = 20000.0 nM 4.70
CHEMBL55479 Ki = 38000.0 nM 4.42