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Assay Detail

CHEMBL751504

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]ouabain from dog kidney Na+/K+ ATPase
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Canis lupus familiaris
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Sodium/potassium-transporting ATPase (CHEMBL2095186)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

Synthesis and quantitative structure-activity relationship of 17 beta-(hydrazonomethyl)-5 beta-androstane-3 beta,14 beta-diol derivatives that bind to Na+,K(+)-ATPase receptor.
Quadri L, Cerri A, Ferrari P, Folpini E, Mabilia M, Melloni P.
J Med Chem (1996) 39 : 3385 -3393
PubMed 8765522 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 5.63 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2068885 1 7.00
CHEMBL2068930 1 6.70
CHEMBL2068942 1 6.70
CHEMBL2068992 1 6.60
CHEMBL2068928 1 6.00
CHEMBL2069000 1 5.70
CHEMBL2068993 1 5.70
CHEMBL2069002 1 5.20
CHEMBL2068932 1 5.10
CHEMBL2068931 1 4.70
CHEMBL2068933 1 4.70
CHEMBL2068997 1 4.60
CHEMBL2069104 1 4.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2068885 IC50 = 100.0 nM 7.00
CHEMBL2068930 IC50 = 199.53 nM 6.70
CHEMBL2068942 IC50 = 199.53 nM 6.70
CHEMBL2068992 IC50 = 251.19 nM 6.60
CHEMBL2068928 IC50 = 1000.0 nM 6.00
CHEMBL2069000 IC50 = 1995.26 nM 5.70
CHEMBL2068993 IC50 = 1995.26 nM 5.70
CHEMBL2069002 IC50 = 6309.57 nM 5.20
CHEMBL2068932 IC50 = 7943.28 nM 5.10
CHEMBL2068931 IC50 = 19952.62 nM 4.70
CHEMBL2068933 IC50 = 19952.62 nM 4.70
CHEMBL2068997 IC50 = 25118.86 nM 4.60
CHEMBL2069104 IC50 = 31622.78 nM 4.50