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Assay Detail

CHEMBL755329

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity for Nicotinic acetylcholine receptor alpha4-beta2 by displacement of [3H](-)-cytisine from whole rat brain membranes
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Synthesis and structure-activity relationships of pyrrolidine-modified analogs of the potent cholinergic channel activator, ABT 418
Lin N, He Y, David J. A, Wasicak JT, Kasson R, Sweeny D, James P. S
Bioorg Med Chem Lett (1994) 4 : 2389 -2394
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 6.13 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL274525 ABT-418 1 8.52
CHEMBL76773 1 7.15
CHEMBL80575 1 6.08
CHEMBL77175 1 5.99
CHEMBL76631 1 5.46
CHEMBL80497 1 5.44
CHEMBL80526 1 5.30
CHEMBL76999 1 5.10
CHEMBL308341 1 -
CHEMBL75870 1 -
CHEMBL308268 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL274525 ABT-418 Ki = 3.0 nM 8.52
CHEMBL76773 Ki = 71.0 nM 7.15
CHEMBL80575 Ki = 840.0 nM 6.08
CHEMBL77175 Ki = 1020.0 nM 5.99
CHEMBL76631 Ki = 3500.0 nM 5.46
CHEMBL80497 Ki = 3666.0 nM 5.44
CHEMBL80526 Ki = 5000.0 nM 5.30
CHEMBL76999 Ki = 8000.0 nM 5.10
CHEMBL308341 Ki > 10000.0 nM -
CHEMBL75870 Ki > 10000.0 nM -
CHEMBL308268 Ki > 10000.0 nM -