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Assay Detail

CHEMBL755330

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Affinity at alpha4-beta2 nACh receptors in rat brain (minus cerebellum) homogenates.
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Cerebellum
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Binding of nicotine and homoazanicotine analogues at neuronal nicotinic acetylcholinergic (nACh) receptors.
Ferretti G, Dukat M, Giannella M, Piergentili A, Pigini M, Quaglia W, Damaj MI, Martin BR, Glennon RA.
Bioorg Med Chem Lett (2003) 13 : 733 -735
PubMed 12639569 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 23 7.29 10.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6623 EPIBATIDINE 1 10.30
CHEMBL58231 1 9.30
CHEMBL70050 1 9.22
CHEMBL3 NICOTINE 4.0 1 8.89
CHEMBL349623 1 8.48
CHEMBL349250 1 8.14
CHEMBL313631 1 8.11
CHEMBL161944 1 7.96
CHEMBL163042 1 7.77
CHEMBL292114 1 7.66
CHEMBL6200 NORNICOTINE 1.0 1 7.52
CHEMBL163244 1 7.42
CHEMBL163748 1 7.34
CHEMBL356996 1 6.82
CHEMBL329602 1 6.69
CHEMBL343169 1 6.49
CHEMBL163552 1 6.44
CHEMBL141612 1 6.07
CHEMBL160034 1 5.67
CHEMBL351320 1 5.49
CHEMBL423429 MYOSMINE 1 5.48
CHEMBL351446 1 5.27
CHEMBL350748 1 5.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6623 EPIBATIDINE Ki = 0.05 nM 10.30
CHEMBL58231 Ki = 0.5 nM 9.30
CHEMBL70050 Ki = 0.6 nM 9.22
CHEMBL3 NICOTINE Ki = 1.3 nM 8.89
CHEMBL349623 Ki = 3.3 nM 8.48
CHEMBL349250 Ki = 7.3 nM 8.14
CHEMBL313631 Ki = 7.8 nM 8.11
CHEMBL161944 Ki = 11.0 nM 7.96
CHEMBL163042 Ki = 17.0 nM 7.77
CHEMBL292114 Ki = 22.0 nM 7.66
CHEMBL6200 NORNICOTINE Ki = 30.0 nM 7.52
CHEMBL163244 Ki = 38.0 nM 7.42
CHEMBL163748 Ki = 46.0 nM 7.34
CHEMBL356996 Ki = 150.0 nM 6.82
CHEMBL329602 Ki = 206.0 nM 6.69
CHEMBL343169 Ki = 325.0 nM 6.49
CHEMBL163552 Ki = 360.0 nM 6.44
CHEMBL141612 Ki = 850.0 nM 6.07
CHEMBL160034 Ki = 2125.0 nM 5.67
CHEMBL351320 Ki = 3260.0 nM 5.49
CHEMBL423429 MYOSMINE Ki = 3300.0 nM 5.48
CHEMBL351446 Ki = 5420.0 nM 5.27
CHEMBL350748 Ki = 9440.0 nM 5.03