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Assay Detail

CHEMBL755904

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity for NPPF receptor in rat spinal cord membranes using the radioligand [125I]-[Tyr1]NPFF
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Spinal cord
Subcellular Membrane
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Neuropeptide FF receptor 2 (CHEMBL3425)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Structure-activity study of neuropeptide FF: contribution of N-terminal regions to affinity and activity.
Gicquel S, Mazarguil H, Desprat C, Allard M, Devillers JP, Simonnet G, Zajac JM.
J Med Chem (1994) 37 : 3477 -3481
PubMed 7932576 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 20 8.53 9.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL414263 1 9.80
CHEMBL385751 1 9.72
CHEMBL420318 1 9.70
CHEMBL269186 1 9.70
CHEMBL429731 1 9.68
CHEMBL386065 1 9.66
CHEMBL331606 1 9.37
CHEMBL410577 1 9.30
CHEMBL266976 1 9.22
CHEMBL406041 1 9.00
CHEMBL407343 1 8.92
CHEMBL386133 1 8.00
CHEMBL326770 1 7.81
CHEMBL116185 1 7.68
CHEMBL267788 1 7.52
CHEMBL115479 1 7.48
CHEMBL325517 1 7.35
CHEMBL117975 1 7.19
CHEMBL325290 1 7.03
CHEMBL420508 1 6.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL414263 Ki = 0.16 nM 9.80
CHEMBL385751 Ki = 0.19 nM 9.72
CHEMBL420318 Ki = 0.2 nM 9.70
CHEMBL269186 Ki = 0.2 nM 9.70
CHEMBL429731 Ki = 0.21 nM 9.68
CHEMBL386065 Ki = 0.22 nM 9.66
CHEMBL331606 Ki = 0.43 nM 9.37
CHEMBL410577 Ki = 0.5 nM 9.30
CHEMBL266976 Ki = 0.6 nM 9.22
CHEMBL406041 Ki = 1.0 nM 9.00
CHEMBL407343 Ki = 1.2 nM 8.92
CHEMBL386133 Ki = 10.0 nM 8.00
CHEMBL326770 Ki = 15.5 nM 7.81
CHEMBL116185 Ki = 20.9 nM 7.68
CHEMBL267788 Ki = 30.0 nM 7.52
CHEMBL115479 Ki = 33.0 nM 7.48
CHEMBL325517 Ki = 45.0 nM 7.35
CHEMBL117975 Ki = 64.0 nM 7.19
CHEMBL325290 Ki = 94.0 nM 7.03
CHEMBL420508 Ki = 300.0 nM 6.52