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Assay Detail

CHEMBL756123

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to Nicotinic acetylcholine receptor alpha4-beta2 using +/-[3H]epibatidine as radioligand in rat brain
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Synthesis and nicotinic binding studies on enantiopure diazine analogues of the novel (2-chloro-5-pyridyl)-9-azabicyclo[4.2.1]non-2-ene UB-165.
Gohlke H, Gündisch D, Schwarz S, Seitz G, Tilotta MC, Wegge T.
J Med Chem (2002) 45 : 1064 -1072
PubMed 11855986 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 9.17 10.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL275466 1 10.40
CHEMBL274538 1 10.29
CHEMBL10886 1 9.85
CHEMBL3 NICOTINE 4.0 1 9.08
CHEMBL25619 1 8.96
CHEMBL10863 1 7.92
CHEMBL10585 1 7.72
CHEMBL6623 EPIBATIDINE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL275466 Ki = 0.04 nM 10.40
CHEMBL274538 Ki = 0.051 nM 10.29
CHEMBL10886 Ki = 0.14 nM 9.85
CHEMBL3 NICOTINE Ki = 0.84 nM 9.08
CHEMBL25619 Ki = 1.1 nM 8.96
CHEMBL10863 Ki = 12.0 nM 7.92
CHEMBL10585 Ki = 19.0 nM 7.72
CHEMBL6623 EPIBATIDINE Ki = 0.008 nM -