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Assay Detail

CHEMBL759158

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of canine cardiac fraction III cAMP phosphodiesterase (PDE 3) at 1.875 mg/kg intravenous dose
4
Total Activities
4
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Canis lupus familiaris
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Phosphodiesterase 3 (CHEMBL2094125)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Synthesis and cardiotonic activity of a series of substituted 4-alkyl-2(1H)-quinazolinones.
Bandurco VT, Schwender CF, Bell SC, Combs DW, Kanojia RM, Levine SD, Mulvey DM, Appollina MA, Reed MS, Malloy EA.
J Med Chem (1987) 30 : 1421 -1426
PubMed 3039135 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.62 4.89

Assay Parameters

Parameter Type Value Units Text
DOSE DOSE 1.875 mg.kg-1
ROUTE ROUTE - Intravenous

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL30001 1 4.89
CHEMBL30387 1 4.75
CHEMBL12856 INAMRINONE 4.0 1 4.22
CHEMBL435758 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL30001 IC50 = 13000.0 nM 4.89
CHEMBL30387 IC50 = 18000.0 nM 4.75
CHEMBL12856 INAMRINONE IC50 = 60000.0 nM 4.22
CHEMBL435758 IC50 = 300000.0 nM -