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Assay Detail

CHEMBL760947

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against phosphodiesterase 1/5 (PDE1/5) extracted from guinea pig trachea smooth muscle cells by using radioligands [3H]-cAMP or [3H]cGMP
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Tissue Trachea
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Phosphodiesterase, PDE1/PDE5 (CHEMBL2097161)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of 4-oxo-1-phenyl-3,4,6,7-tetrahydro-[1,4]diazepino[6,7,1-hi]indoles: novel PDE4 inhibitors.
Pascal Y, Andrianjara CR, Auclair E, Avenel N, Bertin B, Calvet A, Féru F, Lardon S, Moodley I, Ouagued M, Payne A, Pruniaux MP, Szilagyi C.
Bioorg Med Chem Lett (2000) 10 : 35 -38
PubMed 10636238 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 4.14 4.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL332853 1 4.40
CHEMBL126581 1 4.18
CHEMBL339749 1 4.14
CHEMBL338657 1 4.00
CHEMBL340860 1 4.00
CHEMBL333585 1 -
CHEMBL420335 1 -
CHEMBL123266 1 -
CHEMBL419791 1 -
CHEMBL125123 1 -
CHEMBL123782 1 -
CHEMBL339408 1 -
CHEMBL125828 1 -
CHEMBL111925 1 -
CHEMBL341304 1 -
CHEMBL332854 1 -
CHEMBL125671 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL332853 IC50 = 40000.0 nM 4.40
CHEMBL126581 IC50 = 65300.0 nM 4.18
CHEMBL339749 IC50 = 71700.0 nM 4.14
CHEMBL338657 IC50 = 100000.0 nM 4.00
CHEMBL340860 IC50 = 100000.0 nM 4.00
CHEMBL333585 IC50 > 100000.0 nM -
CHEMBL420335 IC50 > 100000.0 nM -
CHEMBL123266 IC50 > 100000.0 nM -
CHEMBL419791 IC50 > 100000.0 nM -
CHEMBL125123 IC50 > 100000.0 nM -
CHEMBL123782 IC50 > 100000.0 nM -
CHEMBL339408 IC50 > 100000.0 nM -
CHEMBL125828 IC50 > 100000.0 nM -
CHEMBL111925 IC50 > 100000.0 nM -
CHEMBL341304 IC50 > 100000.0 nM -
CHEMBL332854 IC50 > 100000.0 nM -
CHEMBL125671 IC50 > 100000.0 nM -