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Assay Detail

CHEMBL762509

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Phosphodiesterase 5 from rabbit platelets
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Oryctolagus cuniculus
Confidence 8 — Homologous single protein target
Curated By Expert

Target

cGMP-specific 3',5'-cyclic phosphodiesterase (CHEMBL1827)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Sildenafil (VIAGRATM), a potent and selective inhibitor of type 5 cGMP phosphodiesterase with utility for the treatment of male erectile dysfunction
Terrett NK, Bell AS, Brown D, Ellis P
Bioorg Med Chem Lett (1996) 6 : 1819 -1824
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.95 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL444046 1 8.68
CHEMBL192 SILDENAFIL 4.0 1 8.44
CHEMBL910 1 8.24
CHEMBL53538 LODENAFIL -1.0 1 7.72
CHEMBL54079 1 7.57
CHEMBL52245 1 6.11
CHEMBL52141 1 6.02
CHEMBL300416 1 6.00
CHEMBL52072 1 5.36
CHEMBL431544 1 5.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL444046 IC50 = 2.1 nM 8.68
CHEMBL192 SILDENAFIL IC50 = 3.6 nM 8.44
CHEMBL910 IC50 = 5.7 nM 8.24
CHEMBL53538 LODENAFIL IC50 = 19.0 nM 7.72
CHEMBL54079 IC50 = 27.0 nM 7.57
CHEMBL52245 IC50 = 780.0 nM 6.11
CHEMBL52141 IC50 = 960.0 nM 6.02
CHEMBL300416 IC50 = 1000.0 nM 6.00
CHEMBL52072 IC50 = 4400.0 nM 5.36
CHEMBL431544 IC50 = 4500.0 nM 5.35