Assay Detail
Functional
CHEMBL765968
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Growth inhibition of MTX-resistant subline R30dm-CCRF-CEM Human Leukemia Cell was determined
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | R30dm-CCRF-CEM |
| Confidence | 1 — Organism |
| Curated By | Intermediate |
Publication
Design, synthesis, and biological activities of classical N-[4-[2-(2-amino-4-ethylpyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-l-glutamic acid and its 6-methyl derivative as potential dual inhibitors of thymidylate synthase and dihydrofolate reductase and as potential antitumor agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 7.78 | 7.89 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.89 |
| CHEMBL439973 | — | — | 1 | 7.73 |
| CHEMBL157434 | — | — | 1 | 7.73 |
| CHEMBL154935 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | EC50 | = | 12.8 | nM | 7.89 |
| CHEMBL439973 | — | EC50 | = | 18.5 | nM | 7.73 |
| CHEMBL157434 | — | EC50 | = | 18.5 | nM | 7.73 |
| CHEMBL154935 | — | EC50 | > | 20000.0 | nM | - |