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Assay Detail

CHEMBL766521

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against Hepatitis C RNA dependent RNA polymerase Nonstructural protein 5B (NS5B polymerase) expressed from baculovirus-infected Sf9 insect cells
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepacivirus hominis
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Expert

Target

RNA-directed RNA polymerase (CHEMBL4296320)
Type SINGLE PROTEIN
Organism Hepacivirus hominis

Publication

Identification of N,N-disubstituted phenylalanines as a novel class of inhibitors of hepatitis C NS5B polymerase.
Chan L, Reddy TJ, Proulx M, Das SK, Pereira O, Wang W, Siddiqui A, Yannopoulos CG, Poisson C, Turcotte N, Drouin A, Alaoui-Ismaili MH, Bethell R, Hamel M, L'Heureux L, Bilimoria D, Nguyen-Ba N.
J Med Chem (2003) 46 : 1283 -1285
PubMed 12672227 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 5.36 6.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL24396 1 6.21
CHEMBL23458 1 6.16
CHEMBL25773 1 6.10
CHEMBL430718 1 5.85
CHEMBL24547 1 5.77
CHEMBL24548 1 5.75
CHEMBL24541 1 5.57
CHEMBL24069 1 5.51
CHEMBL24602 1 5.50
CHEMBL23283 1 5.46
CHEMBL283866 1 5.24
CHEMBL280861 1 5.22
CHEMBL24489 1 5.10
CHEMBL23164 1 5.10
CHEMBL280862 1 5.10
CHEMBL278785 1 5.07
CHEMBL278580 1 5.07
CHEMBL279214 1 5.04
CHEMBL26052 1 4.96
CHEMBL283833 1 4.96
CHEMBL280721 1 4.82
CHEMBL276969 1 4.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL24396 IC50 = 620.0 nM 6.21
CHEMBL23458 IC50 = 700.0 nM 6.16
CHEMBL25773 IC50 = 800.0 nM 6.10
CHEMBL430718 IC50 = 1400.0 nM 5.85
CHEMBL24547 IC50 = 1700.0 nM 5.77
CHEMBL24548 IC50 = 1800.0 nM 5.75
CHEMBL24541 IC50 = 2700.0 nM 5.57
CHEMBL24069 IC50 = 3100.0 nM 5.51
CHEMBL24602 IC50 = 3200.0 nM 5.50
CHEMBL23283 IC50 = 3500.0 nM 5.46
CHEMBL283866 IC50 = 5700.0 nM 5.24
CHEMBL280861 IC50 = 6000.0 nM 5.22
CHEMBL24489 IC50 = 8000.0 nM 5.10
CHEMBL23164 IC50 = 8000.0 nM 5.10
CHEMBL280862 IC50 = 8000.0 nM 5.10
CHEMBL278785 IC50 = 8600.0 nM 5.07
CHEMBL278580 IC50 = 8600.0 nM 5.07
CHEMBL279214 IC50 = 9100.0 nM 5.04
CHEMBL26052 IC50 = 11000.0 nM 4.96
CHEMBL283833 IC50 = 11000.0 nM 4.96
CHEMBL280721 IC50 = 15000.0 nM 4.82
CHEMBL276969 IC50 = 53000.0 nM 4.28