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Assay Detail

CHEMBL771468

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity to prevent binding of added ppp5'A2'p5'A2'pA2'p5'A3'[32P]p5' (c3 label) to RNase L in mouse L cells
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

2-5A-dependent ribonuclease (CHEMBL2687)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Replacement of the ribofuranose oxygen of 2-5A derivatives by methylene: synthesis of an aristeromycin analogue of 2-5A core 5'-monophosphate (5'-O-phosphoryladenylyl)(2'----5')adenyl(2'----5')adenosine.
Sawai H, Lesiak K, Imai J, Torrence PF.
J Med Chem (1985) 28 : 1376 -1380
PubMed 2993624 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.76 9.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL414948 1 9.02
CHEMBL1159914 1 6.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL414948 IC50 = 0.95 nM 9.02
CHEMBL1159914 IC50 = 320.0 nM 6.50