Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL771473

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Increase in intracellular cGMP concentration in atrial natriuretic factor treated rat aortic smooth muscle cells.
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Tissue Cardiac atrium
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

The discovery of tadalafil: a novel and highly selective PDE5 inhibitor. 1: 5,6,11,11a-tetrahydro-1H-imidazo[1',5':1,6]pyrido[3,4-b]indole-1,3(2H)-dione analogues.
Daugan A, Grondin P, Ruault C, Le Monnier de Gouville AC, Coste H, Kirilovsky J, Hyafil F, Labaudinière R.
J Med Chem (2003) 46 : 4525 -4532
PubMed 14521414 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 20 5.88 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL141631 1 7.00
CHEMBL141918 1 6.82
CHEMBL138658 1 6.52
CHEMBL140828 1 6.40
CHEMBL422104 1 6.16
CHEMBL344173 1 6.00
CHEMBL337587 1 6.00
CHEMBL140874 1 5.82
CHEMBL334910 1 5.82
CHEMBL344630 1 5.82
CHEMBL336457 1 5.70
CHEMBL138256 1 5.52
CHEMBL141577 1 5.46
CHEMBL141633 1 5.40
CHEMBL423550 1 5.30
CHEMBL337770 1 5.26
CHEMBL344676 1 5.00
CHEMBL139048 1 -
CHEMBL343832 1 -
CHEMBL502020 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL141631 EC50 = 100.0 nM 7.00
CHEMBL141918 EC50 = 150.0 nM 6.82
CHEMBL138658 EC50 = 300.0 nM 6.52
CHEMBL140828 EC50 = 400.0 nM 6.40
CHEMBL422104 EC50 = 700.0 nM 6.16
CHEMBL344173 EC50 = 1000.0 nM 6.00
CHEMBL337587 EC50 = 1000.0 nM 6.00
CHEMBL140874 EC50 = 1500.0 nM 5.82
CHEMBL334910 EC50 = 1500.0 nM 5.82
CHEMBL344630 EC50 = 1500.0 nM 5.82
CHEMBL336457 EC50 = 2000.0 nM 5.70
CHEMBL138256 EC50 = 3000.0 nM 5.52
CHEMBL141577 EC50 = 3500.0 nM 5.46
CHEMBL141633 EC50 = 4000.0 nM 5.40
CHEMBL423550 EC50 = 5000.0 nM 5.30
CHEMBL337770 EC50 = 5500.0 nM 5.26
CHEMBL344676 EC50 = 10000.0 nM 5.00
CHEMBL139048 EC50 > 10000.0 nM -
CHEMBL343832 EC50 > 10000.0 nM -
CHEMBL502020 EC50 > 10000.0 nM -