Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL784398

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity against high affinity rolipram binding site (HARBS) was determined by displacing radioactive [3H]rolipram from brain membrane suspensions of Wistar rats
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Subcellular Membrane
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Phosphodiesterase 4 (CHEMBL2094267)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Synthesis and structure-activity relationships of 4-oxo-1-phenyl-3,4,6,7-tetrahydro-[1,4]diazepino[6,7,1-hi]indoles: novel PDE4 inhibitors.
Pascal Y, Andrianjara CR, Auclair E, Avenel N, Bertin B, Calvet A, Féru F, Lardon S, Moodley I, Ouagued M, Payne A, Pruniaux MP, Szilagyi C.
Bioorg Med Chem Lett (2000) 10 : 35 -38
PubMed 10636238 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.13 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL63 ROLIPRAM 2.0 1 8.30
CHEMBL125123 1 5.96
CHEMBL332854 1 -
CHEMBL419791 1 -
CHEMBL339749 1 -
CHEMBL332853 1 -
CHEMBL125828 1 -
CHEMBL126581 1 -
CHEMBL111925 1 -
CHEMBL338684 1 -
CHEMBL341304 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL63 ROLIPRAM IC50 = 5.0 nM 8.30
CHEMBL125123 IC50 = 1090.0 nM 5.96
CHEMBL332854 IC50 > 3000.0 nM -
CHEMBL419791 IC50 > 3000.0 nM -
CHEMBL339749 IC50 > 3000.0 nM -
CHEMBL332853 IC50 > 3000.0 nM -
CHEMBL125828 IC50 > 3000.0 nM -
CHEMBL126581 IC50 > 3000.0 nM -
CHEMBL111925 IC50 > 3000.0 nM -
CHEMBL338684 IC50 > 3000.0 nM -
CHEMBL341304 IC50 > 2000.0 nM -