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Assay Detail

CHEMBL800921

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human Sphingosine 1-phosphate receptor 1 expressed in CHO cells was determined by using [33P]-S1P as radioligand
27
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Sphingosine 1-phosphate receptor 1 (CHEMBL4333)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of 3-(N-alkyl)aminopropylphosphonic acids as potent S1P receptor agonists.
Hale JJ, Doherty G, Toth L, Li Z, Mills SG, Hajdu R, Ann Keohane C, Rosenbach M, Milligan J, Shei GJ, Chrebet G, Bergstrom J, Card D, Rosen H, Mandala S.
Bioorg Med Chem Lett (2004) 14 : 3495 -3499
PubMed 15177460 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 8.05 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL115713 1 10.00
CHEMBL118860 2 9.70
CHEMBL117130 1 9.52
CHEMBL119349 1 8.96
CHEMBL432809 1 8.70
CHEMBL334213 1 8.64
CHEMBL118265 1 8.60
CHEMBL119233 1 8.28
CHEMBL325193 1 8.20
CHEMBL117973 1 8.18
CHEMBL115738 1 8.15
CHEMBL432632 1 8.11
CHEMBL325198 1 8.08
CHEMBL1160958 1 7.92
CHEMBL116140 1 7.85
CHEMBL117031 1 7.82
CHEMBL119110 1 7.72
CHEMBL118068 1 7.66
CHEMBL119760 1 7.60
CHEMBL119562 1 7.52
CHEMBL424254 1 7.39
CHEMBL119516 1 7.36
CHEMBL334144 1 7.07
CHEMBL421234 1 6.82
CHEMBL119257 1 6.44
CHEMBL117569 1 5.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL115713 IC50 = 0.1 nM 10.00
CHEMBL118860 IC50 = 0.2 nM 9.70
CHEMBL117130 IC50 = 0.3 nM 9.52
CHEMBL118860 IC50 = 0.6 nM 9.22
CHEMBL119349 IC50 = 1.1 nM 8.96
CHEMBL432809 IC50 = 2.0 nM 8.70
CHEMBL334213 IC50 = 2.3 nM 8.64
CHEMBL118265 IC50 = 2.5 nM 8.60
CHEMBL119233 IC50 = 5.3 nM 8.28
CHEMBL325193 IC50 = 6.3 nM 8.20
CHEMBL117973 IC50 = 6.6 nM 8.18
CHEMBL115738 IC50 = 7.0 nM 8.15
CHEMBL432632 IC50 = 7.7 nM 8.11
CHEMBL325198 IC50 = 8.4 nM 8.08
CHEMBL1160958 IC50 = 12.0 nM 7.92
CHEMBL116140 IC50 = 14.0 nM 7.85
CHEMBL117031 IC50 = 15.0 nM 7.82
CHEMBL119110 IC50 = 19.0 nM 7.72
CHEMBL118068 IC50 = 22.0 nM 7.66
CHEMBL119760 IC50 = 25.0 nM 7.60
CHEMBL119562 IC50 = 30.0 nM 7.52
CHEMBL424254 IC50 = 41.0 nM 7.39
CHEMBL119516 IC50 = 44.0 nM 7.36
CHEMBL334144 IC50 = 85.0 nM 7.07
CHEMBL421234 IC50 = 150.0 nM 6.82
CHEMBL119257 IC50 = 360.0 nM 6.44
CHEMBL117569 IC50 = 1600.0 nM 5.80