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Assay Detail

CHEMBL801614

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Effective concentration that reduces HIV-induced cytopathic effect by 50% was determined by reverse transcriptase (RT) assay.
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 reverse transcriptase (CHEMBL247)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Synthesis and anti-HIV evaluation of D4T and D4T 5'-monophosphate prodrugs.
Sergheraert C, Pierlot C, Tartar A, Henin Y, Lemaitre M.
J Med Chem (1993) 36 : 826 -830
PubMed 8385224 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 7.39 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL129 ZIDOVUDINE 4.0 1 8.00
CHEMBL440551 1 7.70
CHEMBL166784 1 7.70
CHEMBL170331 1 7.40
CHEMBL991 STAVUDINE 4.0 1 7.30
CHEMBL352398 1 7.30
CHEMBL353179 1 7.30
CHEMBL166614 1 7.10
CHEMBL170006 1 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL129 ZIDOVUDINE EC50 = 10.0 nM 8.00
CHEMBL440551 EC50 = 20.0 nM 7.70
CHEMBL166784 EC50 = 20.0 nM 7.70
CHEMBL170331 EC50 = 40.0 nM 7.40
CHEMBL991 STAVUDINE EC50 = 50.0 nM 7.30
CHEMBL352398 EC50 = 50.0 nM 7.30
CHEMBL353179 EC50 = 50.0 nM 7.30
CHEMBL166614 EC50 = 80.0 nM 7.10
CHEMBL170006 EC50 = 200.0 nM 6.70