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Assay Detail

CHEMBL809260

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibition of human thymidylate synthase (TS)
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Thymidylate synthase (CHEMBL1952)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Side chain modified 5-deazafolate and 5-deazatetrahydrofolate analogues as mammalian folylpolyglutamate synthetase and glycinamide ribonucleotide formyltransferase inhibitors: synthesis and in vitro biological evaluation.
Rosowsky A, Forsch RA, Reich VE, Freisheim JH, Moran RG.
J Med Chem (1992) 35 : 1578 -1588
PubMed 1578484 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.14 7.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1160054 1 7.17
CHEMBL39175 1 4.26
CHEMBL294973 1 4.00
CHEMBL38902 1 -
CHEMBL40850 1 -
CHEMBL417696 1 -
CHEMBL41459 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1160054 IC50 = 68.0 nM 7.17
CHEMBL39175 IC50 = 55000.0 nM 4.26
CHEMBL294973 IC50 = 100000.0 nM 4.00
CHEMBL38902 IC50 = 4200000.0 nM -
CHEMBL40850 IC50 = 300000.0 nM -
CHEMBL417696 IC50 = 2100000.0 nM -
CHEMBL41459 IC50 > 5000000.0 nM -