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Assay Detail

CHEMBL812774

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Concentration required for 50% inhibition against thymidylate synthase of Lactobacillus casei
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lacticaseibacillus casei
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Folate analogues. 21. Synthesis and antifolate and antitumor activities of N10-(cyanomethyl)-5,8-dideazafolic acid.
Nair MG, Salter DC, Kisliuk RL, Gaumont Y, North G, Sirotnak FM.
J Med Chem (1983) 26 : 605 -607
PubMed 6403710 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.10 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL422395 1 7.89
CHEMBL34918 1 6.19
CHEMBL34259 METHOTREXATE 4.0 1 4.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL422395 IC50 = 13.0 nM 7.89
CHEMBL34918 IC50 = 640.0 nM 6.19
CHEMBL34259 METHOTREXATE IC50 = 60000.0 nM 4.22