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Assay Detail

CHEMBL815565

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory constant of thymidylate synthase was determined in human AML cells
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type AML
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Thymidylate synthase (CHEMBL1952)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Folate analogues as inhibitors of thymidylate synthase.
Brixner DI, Ueda T, Cheng YC, Hynes JB, Broom AD.
J Med Chem (1987) 30 : 675 -678
PubMed 3470522 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 6.18 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL389051 1 8.15
CHEMBL158990 METHOPTERINE 1 6.00
CHEMBL1622 FOLIC ACID 4.0 1 4.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL389051 Ki = 7.0 nM 8.15
CHEMBL158990 METHOPTERINE Ki = 1000.0 nM 6.00
CHEMBL1622 FOLIC ACID Ki = 40000.0 nM 4.40