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Assay Detail

CHEMBL817338

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of purified human thymidylate synthase from a SV40-transformed human fibroblast cell line cloned in Escherichia coli
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type Fibroblast
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Thymidylate synthase (CHEMBL1952)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Folate analogues. 32. Synthesis and biological evaluation of 2-desamino-2-methyl-N10-propargyl-5,8-dideazafolic acid and related compounds.
Patil SD, Jones C, Nair MG, Galivan J, Maley F, Kisliuk RL, Gaumont Y, Duch D, Ferone R.
J Med Chem (1989) 32 : 1284 -1289
PubMed 2542557 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.07 8.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL25889 1 8.01
CHEMBL24599 1 5.24
CHEMBL24701 1 4.95

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL25889 IC50 = 9.8 nM 8.01
CHEMBL24599 IC50 = 5700.0 nM 5.24
CHEMBL24701 IC50 = 11300.0 nM 4.95