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Assay Detail

CHEMBL819283

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Compound was evaluated for cell growth inhibition of human (WI-L2) leukemic cells.
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type WI-L2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis and biological activity of the 2-desamino and 2-desamino-2-methyl analogues of aminopterin and methotrexate.
Rosowsky A, Forsch RA, Moran RG, Freisheim JH.
J Med Chem (1991) 34 : 227 -234
PubMed 1992122 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 7.30 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL376180 AMINOPTERIN 2.0 1 8.15
CHEMBL34259 METHOTREXATE 4.0 1 7.89
CHEMBL307137 1 7.55
CHEMBL430797 1 7.09
CHEMBL74422 1 6.64
CHEMBL419087 1 6.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL376180 AMINOPTERIN IC50 = 7.0 nM 8.15
CHEMBL34259 METHOTREXATE IC50 = 13.0 nM 7.89
CHEMBL307137 IC50 = 28.0 nM 7.55
CHEMBL430797 IC50 = 81.0 nM 7.09
CHEMBL74422 IC50 = 230.0 nM 6.64
CHEMBL419087 IC50 = 330.0 nM 6.48