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Assay Detail

CHEMBL821055

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [125I]MAdCAM-Ig binding to alpha4-beta7 integrin of human RPMI-8866 cells
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type RPMI-8866
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Integrin alpha-4/beta-7 (CHEMBL2095184)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

The discovery of sulfonylated dipeptides as potent VLA-4 antagonists.
Hagmann WK, Durette PL, Lanza T, Kevin NJ, de Laszlo SE, Kopka IE, Young D, Magriotis PA, Li B, Lin LS, Yang G, Kamenecka T, Chang LL, Wilson J, MacCoss M, Mills SG, Van Riper G, McCauley E, Egger LA, Kidambi U, Lyons K, Vincent S, Stearns R, Colletti A, Teffera J, Tong S, Fenyk-Melody J, Owens K, Levorse D, Kim P, Schmidt JA, Mumford RA.
Bioorg Med Chem Lett (2001) 11 : 2709 -2713
PubMed 11591507 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.09 9.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL91485 1 9.12
CHEMBL76119 1 7.97
CHEMBL56511 1 7.94
CHEMBL309823 1 7.85
CHEMBL91877 1 7.28
CHEMBL313632 1 7.11
CHEMBL90198 1 7.01
CHEMBL91031 1 6.17
CHEMBL88478 1 6.06
CHEMBL93638 1 6.02
CHEMBL278045 1 5.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL91485 IC50 = 0.75 nM 9.12
CHEMBL76119 IC50 = 10.6 nM 7.97
CHEMBL56511 IC50 = 11.5 nM 7.94
CHEMBL309823 IC50 = 14.2 nM 7.85
CHEMBL91877 IC50 = 52.5 nM 7.28
CHEMBL313632 IC50 = 77.0 nM 7.11
CHEMBL90198 IC50 = 97.1 nM 7.01
CHEMBL91031 IC50 = 669.9 nM 6.17
CHEMBL88478 IC50 = 862.0 nM 6.06
CHEMBL93638 IC50 = 948.8 nM 6.02
CHEMBL278045 IC50 = 3551.1 nM 5.45