Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL821384

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory constant for murine Wild-type dihydrofolate reductase (DHFR)
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL4564)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Dihydrofolate reductase mutant with exceptional resistance to methotrexate but not to trimetrexate.
Pineda P, Kanter A, McIvor RS, Benkovic SJ, Rosowsky A, Wagner CR.
J Med Chem (2003) 46 : 2816 -2818
PubMed 12825924 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 10.46 10.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL406356 1 10.74
CHEMBL328499 1 10.48
CHEMBL18155 1 10.38
CHEMBL119 TRIMETREXATE 4.0 1 10.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL406356 Ki = 0.018 nM 10.74
CHEMBL328499 Ki = 0.033 nM 10.48
CHEMBL18155 Ki = 0.042 nM 10.38
CHEMBL119 TRIMETREXATE Ki = 0.06 nM 10.22