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Assay Detail

CHEMBL823759

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]flumazenil binding to rat alpha-3-beta-2-gamma-2 GABA-A receptor subunits expressed in HEK293 cells
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Expert

Target

GABA A receptor alpha-3/beta-2/gamma-2 (CHEMBL2111343)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Novel N-(arylalkyl)indol-3-ylglyoxylylamides targeted as ligands of the benzodiazepine receptor: synthesis, biological evaluation, and molecular modeling analysis of the structure-activity relationships.
Primofiore G, Settimo FD, Taliani S, Marini AM, Novellino E, Greco G, Lavecchia A, Besnard F, Trincavelli L, Costa B, Martini C.
J Med Chem (2001) 44 : 2286 -2297
PubMed 11428922 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 6.08 6.86

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL75306 1 6.86
CHEMBL75456 1 6.55
CHEMBL74302 1 5.80
CHEMBL521901 1 5.71
CHEMBL31796 1 5.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL75306 Ki = 137.0 nM 6.86
CHEMBL75456 Ki = 283.0 nM 6.55
CHEMBL74302 Ki = 1600.0 nM 5.80
CHEMBL521901 Ki = 1960.0 nM 5.71
CHEMBL31796 Ki = 3200.0 nM 5.50