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Assay Detail

CHEMBL824049

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity for mutant rat GABA-A receptor alpha-6-(his,thr,gly,val)beta2gamma2 subunits expressed in HEK293 cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Expert

Target

GABA A receptor alpha-6/beta-2/gamma-2 (CHEMBL2111365)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Four amino acid exchanges convert a diazepam-insensitive, inverse agonist-preferring GABAA receptor into a diazepam-preferring GABAA receptor.
Wieland HA, Lüddens H.
J Med Chem (1994) 37 : 4576 -4580
PubMed 7799410 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 7.03 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL12 DIAZEPAM 4.0 1 8.68
CHEMBL407 FLUMAZENIL 4.0 1 7.48
CHEMBL13662 CI-218872 1 6.11
CHEMBL911 ZOLPIDEM 4.0 1 5.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL12 DIAZEPAM Ki = 2.1 nM 8.68
CHEMBL407 FLUMAZENIL Ki = 33.0 nM 7.48
CHEMBL13662 CI-218872 Ki = 780.0 nM 6.11
CHEMBL911 ZOLPIDEM Ki = 1400.0 nM 5.85