Assay Detail
Functional
CHEMBL827824
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of [3H]norepinephrine uptake in HEK293 cells expressing human norepinephrine transporter
5
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Expert |
Target
Sodium-dependent noradrenaline transporter (CHEMBL222) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis and pharmacology of ethylphenidate enantiomers: the human transesterification metabolite of methylphenidate and ethanol.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 6.62 | 7.29 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1722 | METHYLPHENIDATE HYDROCHLORIDE | 4.0 | 1 | 7.29 |
| CHEMBL2093958 | — | — | 2 | 6.54 |
| CHEMBL370805 | COCAINE | 4.0 | 1 | 6.33 |
| CHEMBL534479 | — | — | 1 | 6.32 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1722 | METHYLPHENIDATE HYDROCHLORIDE | IC50 | = | 51.0 | nM | 7.29 |
| CHEMBL2093958 | — | IC50 | = | 290.0 | nM | 6.54 |
| CHEMBL370805 | COCAINE | IC50 | = | 462.0 | nM | 6.33 |
| CHEMBL534479 | — | IC50 | = | 480.0 | nM | 6.32 |
| CHEMBL2093958 | — | IC50 | > | 10000.0 | nM | - |