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Assay Detail

CHEMBL827846

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory concentration against aggrecanase from porcine chondrocytes using [35S]-sulfate (5 mCi/ml) as radioligand and adding IL-1alpha (5 ng/ml) incubated for 10h
20
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of 3-OH-3-methylpipecolic hydroxamates: potent orally active inhibitors of aggrecanase and MMP-13.
Noe MC, Natarajan V, Snow SL, Wolf-Gouveia LA, Mitchell PG, Lopresti-Morrow L, Reeves LM, Yocum SA, Otterness I, Bliven MA, Carty TJ, Barberia JT, Sweeney FJ, Liras JL, Vaughn M.
Bioorg Med Chem Lett (2005) 15 : 3385 -3388
PubMed 15953722 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.49 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL187092 1 8.82
CHEMBL183474 1 8.43
CHEMBL365283 1 8.33
CHEMBL186663 1 8.16
CHEMBL360854 1 8.10
CHEMBL363126 1 7.96
CHEMBL182306 1 7.85
CHEMBL187474 1 7.82
CHEMBL186500 1 7.82
CHEMBL436277 1 7.77
CHEMBL446697 1 7.72
CHEMBL186743 1 7.29
CHEMBL187245 1 7.11
CHEMBL369115 1 7.07
CHEMBL183995 2 6.92
CHEMBL435102 1 6.31
CHEMBL365658 1 6.30
CHEMBL364643 1 5.70
CHEMBL185168 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL187092 IC50 = 1.5 nM 8.82
CHEMBL183474 IC50 = 3.7 nM 8.43
CHEMBL365283 IC50 = 4.7 nM 8.33
CHEMBL186663 IC50 = 6.9 nM 8.16
CHEMBL360854 IC50 = 7.9 nM 8.10
CHEMBL363126 IC50 = 11.0 nM 7.96
CHEMBL182306 IC50 = 14.0 nM 7.85
CHEMBL187474 IC50 = 15.0 nM 7.82
CHEMBL186500 IC50 = 15.0 nM 7.82
CHEMBL436277 IC50 = 17.0 nM 7.77
CHEMBL446697 IC50 = 19.0 nM 7.72
CHEMBL186743 IC50 = 51.0 nM 7.29
CHEMBL187245 IC50 = 78.0 nM 7.11
CHEMBL369115 IC50 = 85.0 nM 7.07
CHEMBL183995 IC50 = 120.0 nM 6.92
CHEMBL183995 IC50 = 120.0 nM 6.92
CHEMBL435102 IC50 = 490.0 nM 6.31
CHEMBL365658 IC50 = 500.0 nM 6.30
CHEMBL364643 IC50 = 2000.0 nM 5.70
CHEMBL185168 IC50 > 1000.0 nM -