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Assay Detail

CHEMBL829039

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity towards cloned human Gonadotropin-releasing hormone receptor expressed in RBL cells was determined by using [125I]GnRH as radioligand
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type RBL
Confidence 9 — Direct single protein target
Curated By Expert

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of uracil derived human GnRH receptor antagonists: (R)-3-[2-(2-amino)phenethyl]-1-(2,6-difluorobenzyl)-6-methyluracils containing a substituted thiophene or thiazole at C-5.
Rowbottom MW, Tucci FC, Connors PJ, Gross TD, Zhu YF, Guo Z, Moorjani M, Acevedo O, Carter L, Sullivan SK, Xie Q, Fisher A, Struthers RS, Saunders J, Chen C.
Bioorg Med Chem Lett (2004) 14 : 4967 -4973
PubMed 15341961 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 31 7.34 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL363759 1 8.70
CHEMBL365012 1 8.52
CHEMBL112466 1 8.30
CHEMBL185143 1 8.15
CHEMBL364842 1 8.05
CHEMBL186563 1 8.00
CHEMBL184345 1 7.85
CHEMBL183938 1 7.82
CHEMBL363731 1 7.75
CHEMBL364840 1 7.70
CHEMBL426058 1 7.58
CHEMBL185154 1 7.52
CHEMBL363900 1 7.48
CHEMBL367812 1 7.47
CHEMBL186646 1 7.46
CHEMBL438201 1 7.38
CHEMBL365001 1 7.38
CHEMBL187243 1 7.33
CHEMBL425178 1 7.31
CHEMBL187217 1 7.31
CHEMBL184855 1 7.16
CHEMBL363659 1 6.92
CHEMBL366127 1 6.89
CHEMBL360625 1 6.89
CHEMBL361513 1 6.82
CHEMBL360363 1 6.80
CHEMBL361313 1 6.68
CHEMBL365695 1 6.57
CHEMBL367806 1 6.30
CHEMBL360384 1 5.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL363759 Ki = 2.0 nM 8.70
CHEMBL365012 Ki = 3.0 nM 8.52
CHEMBL112466 Ki = 5.0 nM 8.30
CHEMBL185143 Ki = 7.0 nM 8.15
CHEMBL364842 Ki = 9.0 nM 8.05
CHEMBL186563 Ki = 10.0 nM 8.00
CHEMBL184345 Ki = 14.0 nM 7.85
CHEMBL183938 Ki = 15.0 nM 7.82
CHEMBL363731 Ki = 18.0 nM 7.75
CHEMBL364840 Ki = 20.0 nM 7.70
CHEMBL426058 Ki = 26.0 nM 7.58
CHEMBL185154 Ki = 30.0 nM 7.52
CHEMBL363900 Ki = 33.0 nM 7.48
CHEMBL367812 Ki = 34.0 nM 7.47
CHEMBL186646 Ki = 35.0 nM 7.46
CHEMBL438201 Ki = 42.0 nM 7.38
CHEMBL365001 Ki = 42.0 nM 7.38
CHEMBL187243 Ki = 47.0 nM 7.33
CHEMBL425178 Ki = 49.0 nM 7.31
CHEMBL187217 Ki = 49.0 nM 7.31
CHEMBL184855 Ki = 70.0 nM 7.16
CHEMBL363659 Ki = 120.0 nM 6.92
CHEMBL366127 Ki = 130.0 nM 6.89
CHEMBL360625 Ki = 130.0 nM 6.89
CHEMBL361513 Ki = 150.0 nM 6.82
CHEMBL360363 Ki = 160.0 nM 6.80
CHEMBL361313 Ki = 210.0 nM 6.68
CHEMBL365695 Ki = 270.0 nM 6.57
CHEMBL367806 Ki = 500.0 nM 6.30
CHEMBL360384 Ki = 1500.0 nM 5.82
CHEMBL367369 Ki = 3000.0 nM 5.52