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Assay Detail

CHEMBL829929

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of [1-beta-3H]-androstenedione binding to human steroid 5-alpha-reductase type I expressed in DU-145 cells at 10 uM
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type DU-145
Confidence 9 — Direct single protein target
Curated By Expert

Target

3-oxo-5-alpha-steroid 4-dehydrogenase 1 (CHEMBL1787)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel C-17-heteroaryl steroidal CYP17 inhibitors/antiandrogens: synthesis, in vitro biological activity, pharmacokinetics, and antitumor activity in the LAPC4 human prostate cancer xenograft model.
Handratta VD, Vasaitis TS, Njar VC, Gediya LK, Kataria R, Chopra P, Newman D, Farquhar R, Guo Z, Qiu Y, Brodie AM.
J Med Chem (2005) 48 : 2972 -2984
PubMed 15828836 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.67 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL710 FINASTERIDE 4.0 1 7.22
CHEMBL77789 1 6.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL710 FINASTERIDE IC50 = 60.0 nM 7.22
CHEMBL77789 IC50 = 770.0 nM 6.11