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Assay Detail

CHEMBL830137

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [20-3H]-phorbol 12,13-dibutyrate (PDBU) from recombinant Protein kinase C alpha (C1a/C1b domain) in presence of phosphatidyl serine
15
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Macrocyclic diacylglycerol-bis-lactones as conformationally constrained analogues of diacylglycerol-lactones. Interactions with protein kinase C.
Kang JH, Kim SY, Lee J, Marquez VE, Lewin NE, Pearce LV, Blumberg PM.
J Med Chem (2004) 47 : 4000 -4007
PubMed 15267238 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 7.36 8.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL96766 1 8.54
CHEMBL362890 1 8.22
CHEMBL185859 1 7.99
CHEMBL187067 1 7.92
CHEMBL359571 1 7.66
CHEMBL184340 1 7.62
CHEMBL186067 1 7.55
CHEMBL2114288 2 7.46
CHEMBL361050 1 7.28
CHEMBL49904 1 7.11
CHEMBL364849 1 6.97
CHEMBL299225 1 6.86
CHEMBL186395 1 6.00
CHEMBL184615 1 5.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL96766 Ki = 2.9 nM 8.54
CHEMBL362890 Ki = 6.07 nM 8.22
CHEMBL185859 Ki = 10.29 nM 7.99
CHEMBL187067 Ki = 12.16 nM 7.92
CHEMBL359571 Ki = 22.0 nM 7.66
CHEMBL184340 Ki = 24.0 nM 7.62
CHEMBL186067 Ki = 28.0 nM 7.55
CHEMBL2114288 Ki = 35.09 nM 7.46
CHEMBL2114288 Ki = 35.0 nM 7.46
CHEMBL361050 Ki = 52.7 nM 7.28
CHEMBL49904 Ki = 77.85 nM 7.11
CHEMBL364849 Ki = 107.2 nM 6.97
CHEMBL299225 Ki = 138.0 nM 6.86
CHEMBL186395 Ki = 1000.0 nM 6.00
CHEMBL184615 Ki = 1800.0 nM 5.75