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Assay Detail

CHEMBL830408

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human dihydrofolate reductase done at 37 degree C in pH 7.4 with compound
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of classical, four-carbon bridged 5-substituted furo[2,3-d]pyrimidine and 6-substituted pyrrolo[2,3-d]pyrimidine analogues as antifolates.
Gangjee A, Zeng Y, McGuire JJ, Kisliuk RL.
J Med Chem (2005) 48 : 5329 -5336
PubMed 16078850 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 8.15 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL225072 PEMETREXED 4.0 1 8.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL225072 PEMETREXED Ki = 7.0 nM 8.15