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Assay Detail

CHEMBL833438

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibitory concentration to inhibit Ser396 phosphorylation of tau, a natural substrate of GSK-3 in SY5Y cells
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SY5Y
Confidence 9 — Direct single protein target
Curated By Expert

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The development of potent and selective bisarylmaleimide GSK3 inhibitors.
Engler TA, Malhotra S, Burkholder TP, Henry JR, Mendel D, Porter WJ, Furness K, Diefenbacher C, Marquart A, Reel JK, Li Y, Clayton J, Cunningham B, McLean J, O'toole JC, Brozinick J, Hawkins E, Misener E, Briere D, Brier RA, Wagner JR, Campbell RM, Anderson BD, Vaughn R, Bennett DB, Meier TI, Cook JA.
Bioorg Med Chem Lett (2005) 15 : 899 -903
PubMed 15686883 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.95 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL181031 BISARYLMALEIMIDE 1 1 9.52
CHEMBL362588 1 8.80
CHEMBL178737 1 8.59
CHEMBL368246 1 8.30
CHEMBL181339 1 8.11
CHEMBL178851 1 8.06
CHEMBL361007 1 7.96
CHEMBL362814 1 7.92
CHEMBL179725 1 7.89
CHEMBL181464 1 7.42
CHEMBL369572 1 7.28
CHEMBL361948 1 6.96
CHEMBL369316 1 6.53

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL181031 BISARYLMALEIMIDE 1 IC50 = 0.3 nM 9.52
CHEMBL362588 IC50 = 1.6 nM 8.80
CHEMBL178737 IC50 = 2.6 nM 8.59
CHEMBL368246 IC50 = 5.0 nM 8.30
CHEMBL181339 IC50 = 7.7 nM 8.11
CHEMBL178851 IC50 = 8.7 nM 8.06
CHEMBL361007 IC50 = 11.0 nM 7.96
CHEMBL362814 IC50 = 12.0 nM 7.92
CHEMBL179725 IC50 = 13.0 nM 7.89
CHEMBL181464 IC50 = 38.0 nM 7.42
CHEMBL369572 IC50 = 53.0 nM 7.28
CHEMBL361948 IC50 = 111.0 nM 6.96
CHEMBL369316 IC50 = 297.0 nM 6.53