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Assay Detail

CHEMBL835628

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Effective concentration for intracellular cAMP accumulation in human melanocortin 5 receptor expressing HEK 293 cells; (N = 4)
13
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Melanocortin receptor 5 (CHEMBL4608)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel 3D pharmacophore of alpha-MSH/gamma-MSH hybrids leads to selective human MC1R and MC3R analogues.
Cai M, Mayorov AV, Cabello C, Stankova M, Trivedi D, Hruby VJ.
J Med Chem (2005) 48 : 1839 -1848
PubMed 15771429 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 7.06 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 9.00
CHEMBL443071 1 8.72
CHEMBL214332 INTERMEDINE 2.0 1 8.09
CHEMBL411359 1 7.09
CHEMBL405282 1 7.09
CHEMBL439691 1 7.00
CHEMBL438920 1 6.70
CHEMBL2310901 1 6.60
CHEMBL437050 2 6.41
CHEMBL2096759 1 6.39
CHEMBL414778 1 6.31
CHEMBL408509 1 6.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE EC50 = 1.0 nM 9.00
CHEMBL443071 EC50 = 1.9 nM 8.72
CHEMBL214332 INTERMEDINE EC50 = 8.1 nM 8.09
CHEMBL411359 EC50 = 82.0 nM 7.09
CHEMBL405282 EC50 = 82.0 nM 7.09
CHEMBL439691 EC50 = 99.0 nM 7.00
CHEMBL438920 EC50 = 200.0 nM 6.70
CHEMBL2310901 EC50 = 250.0 nM 6.60
CHEMBL437050 EC50 = 390.0 nM 6.41
CHEMBL2096759 EC50 = 410.0 nM 6.39
CHEMBL414778 EC50 = 490.0 nM 6.31
CHEMBL437050 EC50 = 550.0 nM 6.26
CHEMBL408509 EC50 = 870.0 nM 6.06