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Assay Detail

CHEMBL852182

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SK-N-MC
Confidence 9 — Direct single protein target
Curated By Expert

Target

Histamine H4 receptor (CHEMBL3759)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of indole and benzimidazole piperazines as histamine H(4) receptor antagonists.
Terzioglu N, van Rijn RM, Bakker RA, De Esch IJ, Leurs R.
Bioorg Med Chem Lett (2004) 14 : 5251 -5256
PubMed 15454206 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 6.05 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL90 HISTAMINE 4.0 1 8.10
CHEMBL129198 JNJ-7777120 1 7.70
CHEMBL128613 1 7.50
CHEMBL186108 1 7.20
CHEMBL185951 1 7.10
CHEMBL186456 1 6.70
CHEMBL187210 1 6.00
CHEMBL185842 1 5.50
CHEMBL362163 1 5.30
CHEMBL365257 1 5.10
CHEMBL186640 1 4.70
CHEMBL185948 1 4.60
CHEMBL364629 1 4.60
CHEMBL185546 1 4.60
CHEMBL185680 1 -
CHEMBL363089 1 -
CHEMBL186575 1 -
CHEMBL187106 1 -
CHEMBL188020 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL90 HISTAMINE Ki = 7.943 nM 8.10
CHEMBL129198 JNJ-7777120 Ki = 19.95 nM 7.70
CHEMBL128613 Ki = 31.62 nM 7.50
CHEMBL186108 Ki = 63.1 nM 7.20
CHEMBL185951 Ki = 79.43 nM 7.10
CHEMBL186456 Ki = 199.53 nM 6.70
CHEMBL187210 Ki = 1000.0 nM 6.00
CHEMBL185842 Ki = 3162.28 nM 5.50
CHEMBL362163 Ki = 5011.87 nM 5.30
CHEMBL365257 Ki = 7943.28 nM 5.10
CHEMBL186640 Ki = 19952.62 nM 4.70
CHEMBL185948 Ki = 25118.86 nM 4.60
CHEMBL364629 Ki = 25118.86 nM 4.60
CHEMBL185546 Ki = 25118.86 nM 4.60
CHEMBL185680 Ki < 100000.0 nM -
CHEMBL363089 Ki < 100000.0 nM -
CHEMBL186575 Ki < 100000.0 nM -
CHEMBL187106 Ki < 100000.0 nM -
CHEMBL188020 Ki < 100000.0 nM -