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Assay Detail

CHEMBL856874

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human DP receptor expressed in HEK293 cells
8
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Prostaglandin D2 receptor (CHEMBL4427)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a potent and selective prostaglandin D2 receptor antagonist, [(3R)-4-(4-chloro-benzyl)-7-fluoro-5-(methylsulfonyl)-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl]-acetic acid (MK-0524).
Sturino CF, O'Neill G, Lachance N, Boyd M, Berthelette C, Labelle M, Li L, Roy B, Scheigetz J, Tsou N, Aubin Y, Bateman KP, Chauret N, Day SH, Lévesque JF, Seto C, Silva JH, Trimble LA, Carriere MC, Denis D, Greig G, Kargman S, Lamontagne S, Mathieu MC, Sawyer N, Slipetz D, Abraham WM, Jones T, McAuliffe M, Piechuta H, Nicoll-Griffith DA, Wang Z, Zamboni R, Young RN, Metters KM.
J Med Chem (2007) 50 : 794 -806
PubMed 17300164 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 8.63 9.24
Kd 1 10.52 10.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL426559 LAROPIPRANT 4.0 2 10.52
CHEMBL385126 1 8.96
CHEMBL426387 1 8.82
CHEMBL207203 1 8.74
CHEMBL383484 1 8.59
CHEMBL221007 DIASTEREOMER 2 1 8.05
CHEMBL387477 1 8.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL426559 LAROPIPRANT Kd = 0.03 nM 10.52
CHEMBL426559 LAROPIPRANT Ki = 0.57 nM 9.24
CHEMBL385126 Ki = 1.1 nM 8.96
CHEMBL426387 Ki = 1.5 nM 8.82
CHEMBL207203 Ki = 1.8 nM 8.74
CHEMBL383484 Ki = 2.6 nM 8.59
CHEMBL221007 DIASTEREOMER 2 Ki = 8.9 nM 8.05
CHEMBL387477 Ki = 9.6 nM 8.02