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Assay Detail

CHEMBL859608

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human TS
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Expert

Target

Thymidylate synthase (CHEMBL1952)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dual inhibitors of thymidylate synthase and dihydrofolate reductase as antitumor agents: design, synthesis, and biological evaluation of classical and nonclassical pyrrolo[2,3-d]pyrimidine antifolates(1).
Gangjee A, Jain HD, Phan J, Lin X, Song X, McGuire JJ, Kisliuk RL.
J Med Chem (2006) 49 : 1055 -1065
PubMed 16451071 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.94 7.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL225180 1 7.27
CHEMBL389051 1 7.14
CHEMBL371860 1 7.05
CHEMBL201941 1 6.64
CHEMBL201617 1 6.62
CHEMBL225071 RALTITREXED 4.0 1 6.42
CHEMBL371666 1 6.00
CHEMBL202176 1 5.96
CHEMBL201618 1 5.58
CHEMBL381723 1 5.57
CHEMBL202337 1 5.25
CHEMBL436761 1 5.25
CHEMBL225072 PEMETREXED 4.0 1 5.02
CHEMBL383829 1 4.80
CHEMBL202098 1 4.58
CHEMBL34259 METHOTREXATE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL225180 IC50 = 54.0 nM 7.27
CHEMBL389051 IC50 = 72.0 nM 7.14
CHEMBL371860 IC50 = 90.0 nM 7.05
CHEMBL201941 IC50 = 230.0 nM 6.64
CHEMBL201617 IC50 = 240.0 nM 6.62
CHEMBL225071 RALTITREXED IC50 = 380.0 nM 6.42
CHEMBL371666 IC50 = 1000.0 nM 6.00
CHEMBL202176 IC50 = 1100.0 nM 5.96
CHEMBL201618 IC50 = 2600.0 nM 5.58
CHEMBL381723 IC50 = 2700.0 nM 5.57
CHEMBL202337 IC50 = 5600.0 nM 5.25
CHEMBL436761 IC50 = 5600.0 nM 5.25
CHEMBL225072 PEMETREXED IC50 = 9500.0 nM 5.02
CHEMBL383829 IC50 = 16000.0 nM 4.80
CHEMBL202098 IC50 = 26000.0 nM 4.58
CHEMBL34259 METHOTREXATE IC50 - -