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Assay Detail

CHEMBL861815

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]Flumazenil from human GABA-Aalpha1 receptor plus beta-2-gamma-2 expressed in HEK293 cells
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA A receptor alpha-1/beta-1/gamma-2 (CHEMBL2111392)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

4-quinolone derivatives: high-affinity ligands at the benzodiazepine site of brain GABA A receptors. synthesis, pharmacology, and pharmacophore modeling.
Lager E, Andersson P, Nilsson J, Pettersson I, Nielsen EØ, Nielsen M, Sterner O, Liljefors T.
J Med Chem (2006) 49 : 2526 -2533
PubMed 16610795 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 8.20 9.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL381059 1 9.57
CHEMBL206071 1 8.39
CHEMBL12 DIAZEPAM 4.0 1 7.92
CHEMBL205257 1 7.85
CHEMBL911 ZOLPIDEM 4.0 1 7.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL381059 Ki = 0.27 nM 9.57
CHEMBL206071 Ki = 4.1 nM 8.39
CHEMBL12 DIAZEPAM Ki = 12.0 nM 7.92
CHEMBL205257 Ki = 14.0 nM 7.85
CHEMBL911 ZOLPIDEM Ki = 53.0 nM 7.28