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Assay Detail

CHEMBL862521

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of HER stimulated human erbB autophosphorylation in MDA-MB-453 cells
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type MDA-MB-453
Confidence 9 — Direct single protein target
Curated By Expert

Target

Receptor tyrosine-protein kinase erbB-2 (CHEMBL1824)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tyrosine kinase inhibitors. 19. 6-Alkynamides of 4-anilinoquinazolines and 4-anilinopyrido[3,4-d]pyrimidines as irreversible inhibitors of the erbB family of tyrosine kinase receptors.
Klutchko SR, Zhou H, Winters RT, Tran TP, Bridges AJ, Althaus IW, Amato DM, Elliott WL, Ellis PA, Meade MA, Roberts BJ, Fry DW, Gonzales AJ, Harvey PJ, Nelson JM, Sherwood V, Han HK, Pace G, Smaill JB, Denny WA, Showalter HD.
J Med Chem (2006) 49 : 1475 -1485
PubMed 16480284 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.76 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL204638 1 9.30
CHEMBL203599 1 9.00
CHEMBL205059 1 8.70
CHEMBL437890 1 8.46
CHEMBL203661 1 8.30
CHEMBL545315 CANERTINIB DIHYDROCHLORIDE 3.0 1 8.13
CHEMBL373207 1 8.12
CHEMBL204085 1 8.12
CHEMBL202424 1 7.70
CHEMBL202556 1 7.66
CHEMBL382073 1 7.64
CHEMBL203644 1 7.62
CHEMBL202398 1 7.50
CHEMBL383499 1 7.44
CHEMBL202411 1 7.37
CHEMBL380669 1 7.19
CHEMBL202425 1 6.94
CHEMBL203645 1 6.77
CHEMBL202621 1 6.76
CHEMBL202421 1 6.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL204638 IC50 = 0.5 nM 9.30
CHEMBL203599 IC50 = 1.0 nM 9.00
CHEMBL205059 IC50 = 2.0 nM 8.70
CHEMBL437890 IC50 = 3.5 nM 8.46
CHEMBL203661 IC50 = 5.0 nM 8.30
CHEMBL545315 CANERTINIB DIHYDROCHLORIDE IC50 = 7.4 nM 8.13
CHEMBL373207 IC50 = 7.5 nM 8.12
CHEMBL204085 IC50 = 7.6 nM 8.12
CHEMBL202424 IC50 = 20.0 nM 7.70
CHEMBL202556 IC50 = 22.0 nM 7.66
CHEMBL382073 IC50 = 23.0 nM 7.64
CHEMBL203644 IC50 = 24.0 nM 7.62
CHEMBL202398 IC50 = 32.0 nM 7.50
CHEMBL383499 IC50 = 36.0 nM 7.44
CHEMBL202411 IC50 = 43.0 nM 7.37
CHEMBL380669 IC50 = 65.0 nM 7.19
CHEMBL202425 IC50 = 116.0 nM 6.94
CHEMBL203645 IC50 = 170.0 nM 6.77
CHEMBL202621 IC50 = 173.0 nM 6.76
CHEMBL202421 IC50 = 320.0 nM 6.50