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Assay Detail

CHEMBL862545

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of NECA-stimulated cAMP accumulation in CHO cells expressing human adenosine A2B receptor
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

Adenosine receptor A2b (CHEMBL255)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological studies of a new series of 5-heteroarylcarbamoylaminopyrazolo[4,3-e]1,2,4-triazolo[1,5-c]pyrimidines as human A3 adenosine receptor antagonists. Influence of the heteroaryl substituent on binding affinity and molecular modeling investigations.
Pastorin G, Da Ros T, Bolcato C, Montopoli C, Moro S, Cacciari B, Baraldi PG, Varani K, Borea PA, Spalluto G.
J Med Chem (2006) 49 : 1720 -1729
PubMed 16509587 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.57 6.61

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL203660 1 6.61
CHEMBL203054 1 6.60
CHEMBL203060 1 6.58
CHEMBL383689 1 6.55
CHEMBL203998 1 6.50
CHEMBL203506 1 -
CHEMBL262987 1 -
CHEMBL381123 1 -
CHEMBL382665 1 -
CHEMBL382357 1 -
CHEMBL204128 1 -
CHEMBL534936 1 -
CHEMBL383714 1 -
CHEMBL534489 1 -
CHEMBL203797 1 -
CHEMBL118923 1 -
CHEMBL204753 1 -
CHEMBL332091 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL203660 IC50 = 244.0 nM 6.61
CHEMBL203054 IC50 = 250.0 nM 6.60
CHEMBL203060 IC50 = 260.0 nM 6.58
CHEMBL383689 IC50 = 280.0 nM 6.55
CHEMBL203998 IC50 = 320.0 nM 6.50
CHEMBL203506 IC50 > 1000.0 nM -
CHEMBL262987 IC50 > 1000.0 nM -
CHEMBL381123 IC50 > 1000.0 nM -
CHEMBL382665 IC50 > 1000.0 nM -
CHEMBL382357 IC50 > 1000.0 nM -
CHEMBL204128 IC50 > 1000.0 nM -
CHEMBL534936 IC50 > 1000.0 nM -
CHEMBL383714 IC50 > 1000.0 nM -
CHEMBL534489 IC50 > 1000.0 nM -
CHEMBL203797 IC50 > 1000.0 nM -
CHEMBL118923 IC50 > 1000.0 nM -
CHEMBL204753 IC50 > 1000.0 nM -
CHEMBL332091 IC50 > 1000.0 nM -