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Assay Detail

CHEMBL863434

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against LS174T human colon cell line after continuous exposure
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type LS174T
Confidence 1 — Organism
Curated By Intermediate

Target

LS174T (CHEMBL614097)
Type CELL-LINE
Organism Homo sapiens

Publication

Synthesis and biological evaluation of novel pyrrolo[2,1-c][1,4]benzodiazepine prodrugs for use in antibody-directed enzyme prodrug therapy.
Masterson LA, Spanswick VJ, Hartley JA, Begent RH, Howard PW, Thurston DE.
Bioorg Med Chem Lett (2006) 16 : 252 -256
PubMed 16290933 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 7.08 9.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL279754 DSB-120 1 9.33
CHEMBL219864 1 9.10
CHEMBL374490 1 7.68
CHEMBL282988 1 5.85
CHEMBL199952 1 5.80
CHEMBL382927 1 4.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL279754 DSB-120 IC50 = 0.47 nM 9.33
CHEMBL219864 IC50 = 0.8 nM 9.10
CHEMBL374490 IC50 = 21.0 nM 7.68
CHEMBL282988 IC50 = 1400.0 nM 5.85
CHEMBL199952 IC50 = 1600.0 nM 5.80
CHEMBL382927 IC50 = 20000.0 nM 4.70