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Assay Detail

CHEMBL864475

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]CFT from human DAT expressed in COS7 cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Expert

Target

Sodium-dependent dopamine transporter (CHEMBL238)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rhodamine-labeled 2beta-carbomethoxy-3beta-(3,4-dichlorophenyl)tropane analogues as high-affinity fluorescent probes for the dopamine transporter.
Cha JH, Zou MF, Adkins EM, Rasmussen SG, Loland CJ, Schoenenberger B, Gether U, Newman AH.
J Med Chem (2005) 48 : 7513 -7516
PubMed 16302792 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 8.08 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL42553 1 9.70
CHEMBL196760 1 7.75
CHEMBL372378 1 7.57
CHEMBL198842 1 7.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL42553 Ki = 0.2 nM 9.70
CHEMBL196760 Ki = 18.0 nM 7.75
CHEMBL372378 Ki = 27.0 nM 7.57
CHEMBL198842 Ki = 50.0 nM 7.30