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Assay Detail

CHEMBL866784

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Activity at rat CaSR in CHO cells assessed as inhibition of calcium ion-induced [3H]inositol phosphate accumulation
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

Extracellular calcium-sensing receptor (CHEMBL2516)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

N1-Benzoyl-N2-[1-(1-naphthyl)ethyl]-trans-1,2-diaminocyclohexanes: Development of 4-chlorophenylcarboxamide (calhex 231) as a new calcium sensing receptor ligand demonstrating potent calcilytic activity.
Kessler A, Faure H, Petrel C, Rognan D, Césario M, Ruat M, Dauban P, Dodd RH.
J Med Chem (2006) 49 : 5119 -5128
PubMed 16913701 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.90 6.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL536975 1 6.60
CHEMBL537870 1 6.48
CHEMBL180672 1 6.34
CHEMBL557567 1 6.05
CHEMBL556781 1 5.77
CHEMBL537427 1 5.58
CHEMBL537426 1 5.27
CHEMBL559185 1 5.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL536975 IC50 = 250.0 nM 6.60
CHEMBL537870 IC50 = 330.0 nM 6.48
CHEMBL180672 IC50 = 460.0 nM 6.34
CHEMBL557567 IC50 = 900.0 nM 6.05
CHEMBL556781 IC50 = 1700.0 nM 5.77
CHEMBL537427 IC50 = 2600.0 nM 5.58
CHEMBL537426 IC50 = 5400.0 nM 5.27
CHEMBL559185 IC50 = 8000.0 nM 5.10