Assay Detail
Functional
CHEMBL868464
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of cell cycle arrest in H1299 cells at G2 phase in presence of 500 nM doxorubicin by FACS assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | NCI-H1299 |
| Confidence | 1 — Organism |
| Curated By | Intermediate |
Publication
Synthesis and biological evaluation of 1-(2,4,5-trisubstituted phenyl)-3-(5-cyanopyrazin-2-yl)ureas as potent Chk1 kinase inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 5 | 6.18 | 6.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL202635 | — | — | 1 | 6.85 |
| CHEMBL206812 | — | — | 1 | 6.24 |
| CHEMBL205449 | — | — | 1 | 5.84 |
| CHEMBL208332 | — | — | 1 | 5.77 |
| CHEMBL206997 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL202635 | — | EC50 | = | 140.0 | nM | 6.85 |
| CHEMBL206812 | — | EC50 | = | 580.0 | nM | 6.24 |
| CHEMBL205449 | — | EC50 | = | 1450.0 | nM | 5.84 |
| CHEMBL208332 | — | EC50 | = | 1710.0 | nM | 5.77 |
| CHEMBL206997 | — | EC50 | > | 10000.0 | nM | - |