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Assay Detail

CHEMBL871523

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PDE5
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Expert

Target

cGMP-specific 3',5'-cyclic phosphodiesterase (CHEMBL1827)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel pyrazolopyrimidopyridazinones with potent and selective phosphodiesterase 5 (PDE5) inhibitory activity as potential agents for treatment of erectile dysfunction.
Giovannoni MP, Vergelli C, Biancalani C, Cesari N, Graziano A, Biagini P, Gracia J, Gavaldà A, Dal Piaz V.
J Med Chem (2006) 49 : 5363 -5371
PubMed 16913726 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.92 8.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL378542 1 8.08
CHEMBL192 SILDENAFIL 4.0 1 7.70
CHEMBL213060 1 7.64
CHEMBL213219 1 7.42
CHEMBL438451 1 7.40
CHEMBL377563 1 7.30
CHEMBL378255 1 7.15
CHEMBL215699 1 7.10
CHEMBL215756 1 7.02
CHEMBL215465 1 7.00
CHEMBL377850 1 7.00
CHEMBL213113 1 6.85
CHEMBL382494 1 6.80
CHEMBL213425 1 6.75
CHEMBL215700 1 6.66
CHEMBL213099 1 6.62
CHEMBL213094 1 6.43
CHEMBL385683 1 6.41
CHEMBL212397 1 6.33
CHEMBL377144 1 5.85
CHEMBL213265 1 5.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL378542 IC50 = 8.3 nM 8.08
CHEMBL192 SILDENAFIL IC50 = 20.0 nM 7.70
CHEMBL213060 IC50 = 23.0 nM 7.64
CHEMBL213219 IC50 = 38.0 nM 7.42
CHEMBL438451 IC50 = 40.0 nM 7.40
CHEMBL377563 IC50 = 50.0 nM 7.30
CHEMBL378255 IC50 = 71.0 nM 7.15
CHEMBL215699 IC50 = 79.0 nM 7.10
CHEMBL215756 IC50 = 95.0 nM 7.02
CHEMBL215465 IC50 = 100.0 nM 7.00
CHEMBL377850 IC50 = 100.0 nM 7.00
CHEMBL213113 IC50 = 140.0 nM 6.85
CHEMBL382494 IC50 = 160.0 nM 6.80
CHEMBL213425 IC50 = 180.0 nM 6.75
CHEMBL215700 IC50 = 220.0 nM 6.66
CHEMBL213099 IC50 = 240.0 nM 6.62
CHEMBL213094 IC50 = 370.0 nM 6.43
CHEMBL385683 IC50 = 390.0 nM 6.41
CHEMBL212397 IC50 = 470.0 nM 6.33
CHEMBL377144 IC50 = 1400.0 nM 5.85
CHEMBL213265 IC50 = 1500.0 nM 5.82