Assay Detail
Binding
CHEMBL875148
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In vitro inhibitory activity against L1210 dihydrofolate reductase in rodent neoplastic cells
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | L1210 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
10-Propargylaminopterin and alkyl homologues of methotrexate as inhibitors of folate metabolism.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 5 | 8.53 | 9.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL166915 | — | — | 1 | 9.30 |
| CHEMBL171757 | — | — | 1 | 7.76 |
| CHEMBL376180 | AMINOPTERIN | 2.0 | 1 | - |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | - |
| CHEMBL354055 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL166915 | — | Ki | = | 0.5 | nM | 9.30 |
| CHEMBL171757 | — | Ki | = | 17.5 | nM | 7.76 |
| CHEMBL376180 | AMINOPTERIN | Ki | = | 0.004 | nM | - |
| CHEMBL34259 | METHOTREXATE | Ki | = | 0.006 | nM | - |
| CHEMBL354055 | — | Ki | = | 0.003 | nM | - |