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Assay Detail

CHEMBL880429

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Concentration required for displacement of [33P]-labeled S1P from human sphingosine 1-phosphate receptor 1 expressed in CHO cells
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

Sphingosine 1-phosphate receptor 1 (CHEMBL4333)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent 3,5-diphenyl-1,2,4-oxadiazole sphingosine-1-phosphate-1 (S1P1) receptor agonists with exceptional selectivity against S1P2 and S1P3.
Li Z, Chen W, Hale JJ, Lynch CL, Mills SG, Hajdu R, Keohane CA, Rosenbach MJ, Milligan JA, Shei GJ, Chrebet G, Parent SA, Bergstrom J, Card D, Forrest M, Quackenbush EJ, Wickham LA, Vargas H, Evans RM, Rosen H, Mandala S.
J Med Chem (2005) 48 : 6169 -6173
PubMed 16190743 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 8.56 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL198976 1 9.40
CHEMBL381872 1 9.40
CHEMBL194419 1 9.22
CHEMBL195014 1 9.10
CHEMBL198415 1 9.05
CHEMBL194578 1 8.89
CHEMBL372066 1 8.89
CHEMBL196534 1 8.85
CHEMBL195025 1 8.77
CHEMBL196357 1 8.74
CHEMBL194898 1 8.66
CHEMBL193789 1 8.54
CHEMBL366181 1 8.42
CHEMBL195141 1 8.42
CHEMBL371985 1 8.35
CHEMBL371743 1 8.29
CHEMBL364899 1 8.09
CHEMBL197502 1 7.57
CHEMBL371670 1 7.54
CHEMBL196149 1 7.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL198976 IC50 = 0.4 nM 9.40
CHEMBL381872 IC50 = 0.4 nM 9.40
CHEMBL194419 IC50 = 0.6 nM 9.22
CHEMBL195014 IC50 = 0.8 nM 9.10
CHEMBL198415 IC50 = 0.9 nM 9.05
CHEMBL194578 IC50 = 1.3 nM 8.89
CHEMBL372066 IC50 = 1.3 nM 8.89
CHEMBL196534 IC50 = 1.4 nM 8.85
CHEMBL195025 IC50 = 1.7 nM 8.77
CHEMBL196357 IC50 = 1.8 nM 8.74
CHEMBL194898 IC50 = 2.2 nM 8.66
CHEMBL193789 IC50 = 2.9 nM 8.54
CHEMBL366181 IC50 = 3.8 nM 8.42
CHEMBL195141 IC50 = 3.8 nM 8.42
CHEMBL371985 IC50 = 4.5 nM 8.35
CHEMBL371743 IC50 = 5.1 nM 8.29
CHEMBL364899 IC50 = 8.2 nM 8.09
CHEMBL197502 IC50 = 27.0 nM 7.57
CHEMBL371670 IC50 = 29.0 nM 7.54
CHEMBL196149 IC50 = 100.0 nM 7.00