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Assay Detail

CHEMBL888163

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Binding
Inhibition of [3H]GABA uptake at mouse GAT1 expressed in D8 cells
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type D8
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Sodium- and chloride-dependent GABA transporter 1 (CHEMBL5445)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and biological evaluation of (R)-N-(diarylmethylthio/sulfinyl)ethyl/propyl-piperidine-3-carboxylic acid hydrochlorides as novel GABA uptake inhibitors.
Zhang J, Zhang P, Liu X, Fang K, Lin G.
Bioorg Med Chem Lett (2007) 17 : 3769 -3773
PubMed 17517506 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.60 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1695 TIAGABINE HYDROCHLORIDE 4.0 1 7.10
CHEMBL538839 1 6.04
CHEMBL558578 1 5.93
CHEMBL538596 1 4.94
CHEMBL536532 1 4.80
CHEMBL541139 1 4.77
CHEMBL537448 1 -
CHEMBL536762 1 -
CHEMBL536534 1 -
CHEMBL536533 1 -
CHEMBL537671 1 -
CHEMBL537449 1 -
CHEMBL539347 1 -
CHEMBL77575 (R)-NIPECOTIC ACID 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1695 TIAGABINE HYDROCHLORIDE IC50 = 80.0 nM 7.10
CHEMBL538839 IC50 = 920.0 nM 6.04
CHEMBL558578 IC50 = 1180.0 nM 5.93
CHEMBL538596 IC50 = 11600.0 nM 4.94
CHEMBL536532 IC50 = 15800.0 nM 4.80
CHEMBL541139 IC50 = 16900.0 nM 4.77
CHEMBL537448 IC50 = 1600000.0 nM -
CHEMBL536762 IC50 = 105000.0 nM -
CHEMBL536534 IC50 = 589000.0 nM -
CHEMBL536533 IC50 = 120000.0 nM -
CHEMBL537671 IC50 = 2130000.0 nM -
CHEMBL537449 IC50 = 4800000.0 nM -
CHEMBL539347 IC50 = 240000.0 nM -
CHEMBL77575 (R)-NIPECOTIC ACID IC50 = 456000.0 nM -