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Assay Detail

CHEMBL891835

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Induction of Hsp90-dependent Her2 protein degradation in Skbr3 cells after 24 hrs by ELISA
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SK-BR-3
Confidence 1 — Organism
Curated By Autocuration

Target

SK-BR-3 (CHEMBL613834)
Type CELL-LINE
Organism Homo sapiens

Publication

High-throughput assay for the identification of Hsp90 inhibitors based on Hsp90-dependent refolding of firefly luciferase.
Galam L, Hadden MK, Ma Z, Ye QZ, Yun BG, Blagg BS, Matts RL.
Bioorg Med Chem (2007) 15 : 1939 -1946
PubMed 17223347 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 4.66 5.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL244858 1 5.36
CHEMBL395021 1 5.33
CHEMBL244645 1 5.05
CHEMBL244649 1 4.96
CHEMBL391976 1 4.90
CHEMBL267604 1 4.67
CHEMBL395576 1 4.48
CHEMBL397442 1 4.36
CHEMBL243378 1 4.29
CHEMBL244857 1 4.21
CHEMBL242071 1 4.19
CHEMBL242070 1 4.17
CHEMBL373839 1 -
CHEMBL244648 1 -
CHEMBL244440 1 -
CHEMBL244859 1 -
CHEMBL243379 1 -
CHEMBL244856 1 -
CHEMBL244644 1 -
CHEMBL388919 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL244858 IC50 = 4400.0 nM 5.36
CHEMBL395021 IC50 = 4700.0 nM 5.33
CHEMBL244645 IC50 = 8900.0 nM 5.05
CHEMBL244649 IC50 = 10900.0 nM 4.96
CHEMBL391976 IC50 = 12500.0 nM 4.90
CHEMBL267604 IC50 = 21300.0 nM 4.67
CHEMBL395576 IC50 = 33000.0 nM 4.48
CHEMBL397442 IC50 = 44000.0 nM 4.36
CHEMBL243378 IC50 = 51600.0 nM 4.29
CHEMBL244857 IC50 = 61000.0 nM 4.21
CHEMBL242071 IC50 = 64000.0 nM 4.19
CHEMBL242070 IC50 = 67000.0 nM 4.17
CHEMBL373839 IC50 > 100000.0 nM -
CHEMBL244648 IC50 > 100000.0 nM -
CHEMBL244440 IC50 > 100000.0 nM -
CHEMBL244859 IC50 > 100000.0 nM -
CHEMBL243379 IC50 > 100000.0 nM -
CHEMBL244856 IC50 > 100000.0 nM -
CHEMBL244644 IC50 > 100000.0 nM -
CHEMBL388919 IC50 > 100000.0 nM -