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Assay Detail

CHEMBL899872

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CL receptor expressed in E10 cells assessed as CGRP-stimulated cAMP production in presence of 50% human serum
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type E10
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Calcitonin gene-related peptide type 1 receptor (CHEMBL3798)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent, orally bioavailable calcitonin gene-related peptide receptor antagonists for the treatment of migraine: discovery of N-[(3R,6S)-6-(2,3-difluorophenyl)-2-oxo-1- (2,2,2-trifluoroethyl)azepan-3-yl]-4- (2-oxo-2,3-dihydro-1H-imidazo[4,5-b]pyridin- 1-yl)piperidine-1-carboxamide (MK-0974).
Paone DV, Shaw AW, Nguyen DN, Burgey CS, Deng JZ, Kane SA, Koblan KS, Salvatore CA, Mosser SD, Johnston VK, Wong BK, Miller-Stein CM, Hershey JC, Graham SL, Vacca JP, Williams TM.
J Med Chem (2007) 50 : 5564 -5567
PubMed 17929795 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.53 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL392636 1 8.70
CHEMBL237662 1 8.52
CHEMBL392634 1 8.52
CHEMBL392730 1 8.30
CHEMBL238276 1 8.22
CHEMBL438120 1 8.10
CHEMBL415263 1 8.10
CHEMBL392937 1 8.00
CHEMBL392635 1 8.00
CHEMBL236593 TELCAGEPANT 3.0 1 7.96
CHEMBL237849 1 7.82
CHEMBL237663 1 7.68
CHEMBL237664 1 7.66
CHEMBL237661 1 7.66
CHEMBL236590 1 7.64
CHEMBL237848 1 7.52
CHEMBL239630 1 6.92
CHEMBL391660 1 6.60
CHEMBL241118 1 6.60
CHEMBL398575 1 6.47
CHEMBL240904 1 6.21
CHEMBL429803 1 6.16
CHEMBL240686 1 5.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL392636 IC50 = 2.0 nM 8.70
CHEMBL237662 IC50 = 3.0 nM 8.52
CHEMBL392634 IC50 = 3.0 nM 8.52
CHEMBL392730 IC50 = 5.0 nM 8.30
CHEMBL238276 IC50 = 6.0 nM 8.22
CHEMBL438120 IC50 = 8.0 nM 8.10
CHEMBL415263 IC50 = 8.0 nM 8.10
CHEMBL392937 IC50 = 10.0 nM 8.00
CHEMBL392635 IC50 = 10.0 nM 8.00
CHEMBL236593 TELCAGEPANT IC50 = 11.0 nM 7.96
CHEMBL237849 IC50 = 15.0 nM 7.82
CHEMBL237663 IC50 = 21.0 nM 7.68
CHEMBL237664 IC50 = 22.0 nM 7.66
CHEMBL237661 IC50 = 22.0 nM 7.66
CHEMBL236590 IC50 = 23.0 nM 7.64
CHEMBL237848 IC50 = 30.0 nM 7.52
CHEMBL239630 IC50 = 120.0 nM 6.92
CHEMBL391660 IC50 = 250.0 nM 6.60
CHEMBL241118 IC50 = 250.0 nM 6.60
CHEMBL398575 IC50 = 340.0 nM 6.47
CHEMBL240904 IC50 = 620.0 nM 6.21
CHEMBL429803 IC50 = 700.0 nM 6.16
CHEMBL240686 IC50 = 1800.0 nM 5.75